第838回 生医研セミナー(多階層生体防御システム研究拠点)
下記の通り、武井 洋大 先生によるセミナーを開催します。皆様のご来聴を心より歓迎致します。
※参加申し込みは不要です。
※セミナーは日本語で行われます。
演題 / Title
High-resolution spatial multi-omics reveals single-cell nuclear architecture in tissues
演者 / Speaker
Dr. Yodai Takei （武井 洋大 先生）
California Institute of Technology, Pasadena, CA, USA.
日時 / Date
２０２３年９月２８日（木） / Sep. 28 (Thu), 2023
17：00〜18：00
場所 / Venue
病院キャンパス コラボ・ステーションⅠ 1階 共同セミナー室A・B
地図の35番です。
要 旨 / Abstract
The mammalian nucleus forms subnuclear compartments marked by diverse subnuclear structures such as nuclear bodies and histone modifications. These subnuclear compartments are often cell-type specific and affect gene regulation and 3D genome organization. To understand nuclear organization and its function, it is critical to identify the molecular constituents of subnuclear compartments and map their associations with specific genomic loci in individual cells, within complex tissues. However, our ability to directly map and understand organization of single nuclei in tissues has been limited due to the lack of high-resolution multi-modal single-cell technology. Here, we introduce a new imaging-based single-cell multi-omics technology, enabling simultaneous in situ mapping of 100,049 genomic loci (two-layer DNA seqFISH+), together with nascent transcriptome for 17,856 genes (intron seqFISH+) and a diverse set of subnuclear structures (sequential immunofluorescence) all in single cells in cell lines and adult mouse cerebellum. These multi-omics datasets contain rich information such as chromosome structures, chromatin profiles, gene expression profiles, physical properties of subnuclear structures from individual cells. Using these datasets, we found that repressive subnuclear compartments are more variable by cell type than active subnuclear compartments. Specifically, we identified cell-type specific repressive compartments, which can shape structural hubs for cell-type specific radial chromosomal positioning and inter-chromosomal interactions in neurons and glial cells. Together, our results provide a major step forward in understanding single-cell nuclear architecture, in the context of subnuclear compartments, associated genomic loci, and their impacts on gene regulation, directly within complex tissues. We anticipate similar approaches can be applicable to various biological samples.
連絡先 / Contact
生体防御医学研究所 トランスクリプトミクス分野
大川 恭行
092 (642) 4534
Division of Transcriptomics, Medical Institute of Bioregulation
Yasuyuki Ohkawa
TEL: 092 (642) 4534