{"id":430,"date":"2025-11-05T00:00:51","date_gmt":"2025-11-05T00:00:51","guid":{"rendered":"https:\/\/bioreg.kyushu-u.ac.jp\/labo\/iden\/?post_type=service&p=430"},"modified":"2025-11-05T05:51:36","modified_gmt":"2025-11-05T05:51:36","slug":"2511050000","status":"publish","type":"service","link":"https:\/\/bioreg.kyushu-u.ac.jp\/labo\/iden\/publication\/2511050000\/","title":{"rendered":"Front. Immunol. 2025 (Koutsogiannaki et al.)"},"content":{"rendered":"\n


DOCK2 as a novel CD11c ligand in neutrophils to regulate reactive oxygen species production<\/p>\n\n\n\n

Koutsogiannaki S, Hou L, Alhamdan F, Mastali M, Murray C, Van Eyk J, Kunimura K<\/strong>, Yuki K:<\/p>\n\n\n\n

Frontiers in Immunology<\/strong> 16: 1692451, 2025<\/strong>. <\/p>\n\n\n\n

Abstract:<\/strong> 
CD11c (integrin \u03b1X) is one of the \u03b2\u2082 integrin members traditionally recognized as a dendritic cell marker. It forms the CD11c\/CD18 heterodimer\u2014also known as complement receptor 4 (CR4)\u2014and mediates ligand binding to complement fragments, fibrinogen, and intercellular adhesion molecules in vitro. Although historically its expression on dendritic cells and a subset of macrophage populations has been well recognized, recent findings reveal that it demonstrates broader expression profile, including in neutrophils. In neutrophils, CD11c is predominantly intracellular, suggesting a non-canonical role beyond cellular adhesion. We previously identified IQGAP1 as an intracellular binding partner of CD11c\/CD18, implicating this interaction in neutrophil maturation. Here mature CD11c-deficient neutrophils displayed impaired reactive oxygen species (ROS) generation while maintaining normal phagocytosis, indicating a selective defect in oxidative burst. Given the central role of NADPH oxidase and Rac activation in ROS production, we hypothesized that CD11c would influence this pathway. Phospho-proteomic profiling revealed reduced phosphorylation of the Rac guanine nucleotide exchange factor DOCK2 in CD11c-deficient neutrophils upon phorbol 12-myristate 13-acetate (PMA) stimulation. The analysis involving immunoprecipitation and proteomics confirmed a CD11c\u2013DOCK2 association. These results supported a model in which CD11c would directly engage DOCK2 to promote Rac activation and NADPH oxidase function, uncovering a novel integrin-mediated mechanism regulating neutrophil effector activity. This work expands the functional repertoire of CD11c and provides a new insight into integrin signaling in innate immunity.<\/p>\n\n\n\n

https:\/\/www.frontiersin.org\/journals\/immunology\/articles\/10.3389\/fimmu.2025.1692451\/full<\/a><\/p>\n\n\n\n

\"\"<\/figure>\n","protected":false},"featured_media":473,"template":"","service_category":[8],"class_list":["post-430","service","type-service","status-publish","has-post-thumbnail","hentry","service_category-original-paper"],"_links":{"self":[{"href":"https:\/\/bioreg.kyushu-u.ac.jp\/labo\/iden\/wp-json\/wp\/v2\/service\/430","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/bioreg.kyushu-u.ac.jp\/labo\/iden\/wp-json\/wp\/v2\/service"}],"about":[{"href":"https:\/\/bioreg.kyushu-u.ac.jp\/labo\/iden\/wp-json\/wp\/v2\/types\/service"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bioreg.kyushu-u.ac.jp\/labo\/iden\/wp-json\/wp\/v2\/media\/473"}],"wp:attachment":[{"href":"https:\/\/bioreg.kyushu-u.ac.jp\/labo\/iden\/wp-json\/wp\/v2\/media?parent=430"}],"wp:term":[{"taxonomy":"service_category","embeddable":true,"href":"https:\/\/bioreg.kyushu-u.ac.jp\/labo\/iden\/wp-json\/wp\/v2\/service_category?post=430"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}