{"id":257,"date":"2025-04-01T00:10:49","date_gmt":"2025-04-01T00:10:49","guid":{"rendered":"https:\/\/bioreg.kyushu-u.ac.jp\/labo\/iden\/wp\/?post_type=service&#038;p=257"},"modified":"2025-07-21T03:30:40","modified_gmt":"2025-07-21T03:30:40","slug":"paper-10","status":"publish","type":"service","link":"https:\/\/bioreg.kyushu-u.ac.jp\/labo\/iden\/publication\/paper-10\/","title":{"rendered":"Allergy 2022 (Kunimura et al.)"},"content":{"rendered":"\n<p>Identification of a functional <em>DOCK8<\/em> gene polymorphism associated with atopic dermatitis.<\/p>\n\n\n\n<p><strong>Kunimura K<\/strong><sup>\u2020<\/sup>, Yamamura K, Nakahara T, Kido-Nakahara M, <strong>Uruno T<\/strong>, <strong>Fukui Y<\/strong> (<sup>\u2020<\/sup>corresponding author):<\/p>\n\n\n\n<p><strong>Allergy<\/strong> 77(12): 3670-3672, <strong>2022<\/strong>.<\/p>\n\n\n\n<p>Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by recurrent eczematous lesions, intense itch, and type 2 immune responses. Interleukin 31 (IL-31) is a cytokine mainly produced from skin-homing CLA+ T cells. Serum IL-31 levels correlate with the disease severity of AD patients, and the administration of an anti-IL-31 receptor antibody alleviates pruritus in AD patients. Thus, IL-31 plays a major role in AD pathogenesis. Dedicator of cytokinesis 8 (DOCK8) is an evolutionarily conserved guanine nucleotide exchange factor (GEF) for Cdc42. Bi-allelic loss-of-function mutations of DOCK8 cause a combined immunodeficiency characterized by AD. We have previously shown that knock down of DOCK8 gene in T cells from healthy controls markedly increases T-cell receptor-mediated IL31 gene expression. Therefore, DOCK8 acts as a negative regulator for IL-31 induction in human T cells. However, DOCK8 expression is comparable between healthy controls and AD patients,6 and functional significance of DOCK8 in the disease predisposition remains unknown. The aim of this study was to evaluate whether DOCK8 gene polymorphism is associated with AD. We recruited age- and gender-matched 46 AD patients and 46 healthy controls. Gene polymorphisms in all exons of DOCK8 (Exon 1\u201348) were analyzed by direct DNA sequencing using exon-specific primers. As a result, we found that DOCK8 polymorphism at position 1790 (rs17673268) is associated with the susceptibility to AD and revealed its underlying molecular basis.<\/p>\n\n\n\n<p><a href=\"https:\/\/researchmap.jp\/-kunimura-\/published_papers\/38176647\">https:\/\/researchmap.jp\/-kunimura-\/published_papers\/38176647<\/a><\/p>\n\n\n\n<figure class=\"wp-block-image size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"523\" src=\"https:\/\/bioreg.kyushu-u.ac.jp\/labo\/iden\/wp\/wp-content\/uploads\/2025\/04\/\u30b9\u30af\u30ea\u30fc\u30f3\u30b7\u30e7\u30c3\u30c8-2025-07-03-13.37.32-1024x523.jpg\" alt=\"\" class=\"wp-image-263\" style=\"width:642px;height:auto\" srcset=\"https:\/\/bioreg.kyushu-u.ac.jp\/labo\/iden\/wp\/wp-content\/uploads\/2025\/04\/\u30b9\u30af\u30ea\u30fc\u30f3\u30b7\u30e7\u30c3\u30c8-2025-07-03-13.37.32-1024x523.jpg 1024w, https:\/\/bioreg.kyushu-u.ac.jp\/labo\/iden\/wp\/wp-content\/uploads\/2025\/04\/\u30b9\u30af\u30ea\u30fc\u30f3\u30b7\u30e7\u30c3\u30c8-2025-07-03-13.37.32-300x153.jpg 300w, https:\/\/bioreg.kyushu-u.ac.jp\/labo\/iden\/wp\/wp-content\/uploads\/2025\/04\/\u30b9\u30af\u30ea\u30fc\u30f3\u30b7\u30e7\u30c3\u30c8-2025-07-03-13.37.32-768x392.jpg 768w, https:\/\/bioreg.kyushu-u.ac.jp\/labo\/iden\/wp\/wp-content\/uploads\/2025\/04\/\u30b9\u30af\u30ea\u30fc\u30f3\u30b7\u30e7\u30c3\u30c8-2025-07-03-13.37.32-1536x785.jpg 1536w, https:\/\/bioreg.kyushu-u.ac.jp\/labo\/iden\/wp\/wp-content\/uploads\/2025\/04\/\u30b9\u30af\u30ea\u30fc\u30f3\u30b7\u30e7\u30c3\u30c8-2025-07-03-13.37.32.jpg 2004w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n","protected":false},"featured_media":263,"template":"","service_category":[8],"class_list":["post-257","service","type-service","status-publish","has-post-thumbnail","hentry","service_category-original-paper"],"_links":{"self":[{"href":"https:\/\/bioreg.kyushu-u.ac.jp\/labo\/iden\/wp-json\/wp\/v2\/service\/257","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/bioreg.kyushu-u.ac.jp\/labo\/iden\/wp-json\/wp\/v2\/service"}],"about":[{"href":"https:\/\/bioreg.kyushu-u.ac.jp\/labo\/iden\/wp-json\/wp\/v2\/types\/service"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bioreg.kyushu-u.ac.jp\/labo\/iden\/wp-json\/wp\/v2\/media\/263"}],"wp:attachment":[{"href":"https:\/\/bioreg.kyushu-u.ac.jp\/labo\/iden\/wp-json\/wp\/v2\/media?parent=257"}],"wp:term":[{"taxonomy":"service_category","embeddable":true,"href":"https:\/\/bioreg.kyushu-u.ac.jp\/labo\/iden\/wp-json\/wp\/v2\/service_category?post=257"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}