{"id":252,"date":"2025-04-01T00:09:55","date_gmt":"2025-04-01T00:09:55","guid":{"rendered":"https:\/\/bioreg.kyushu-u.ac.jp\/labo\/iden\/wp\/?post_type=service&#038;p=252"},"modified":"2025-07-21T03:31:23","modified_gmt":"2025-07-21T03:31:23","slug":"paper-9","status":"publish","type":"service","link":"https:\/\/bioreg.kyushu-u.ac.jp\/labo\/iden\/publication\/paper-9\/","title":{"rendered":"BBRC 2022 (Tatsuguchi, Uruno et al.)"},"content":{"rendered":"\n<p>Pharmacological intervention of cholesterol sulfate-mediated T cell exclusion promotes antitumor immunity.<\/p>\n\n\n\n<p><strong>Tatsuguchi T<\/strong>, <strong>Uruno T<\/strong>, Sugiura Y, Oisaki K, Takaya D, <strong>Sakata D<\/strong>, Izumi Y, Togo T, Hattori Y, <strong>Kunimura K<\/strong>, <strong>Sakurai T<\/strong>, Honma T, Bamba T, Nakamura M, Kanai M, Suematsu M, <strong>Fukui Y<\/strong>:<\/p>\n\n\n\n<p><strong>Biochemical and Biophysical Research Communications<\/strong> 609: 183-188, <strong>2022<\/strong>.<\/p>\n\n\n\n<p>Effective cancer immunotherapy requires physical contact of T cells with cancer cells. However, tumors often constitute special microenvironments that exclude T cells and resist immunotherapy. Cholesterol sulfate (CS) is a product of sulfotransferase SULT2B1b and acts as an endogenous inhibitor of DOCK2, a Rac activator essential for migration and activation of lymphocytes. We have recently shown that cancer-derived CS prevents tumor infiltration by effector T cells. Therefore, SULT2B1b may be a therapeutic target to dampen CS-mediated immune evasion. Here, we identified 3\u03b2-hydroxy-5-cholenoic acid (3\u03b2-OH-5-Chln) as a cell-active inhibitor of SULT2B1b. 3\u03b2-OH-5-Chln inhibited the cholesterol sulfotransferase activity of SULT2B1b in vitro and suppressed CS production from cancer cells expressing SULT2B1b. In vivo administration of 3\u03b2-OH-5-Chln locally reduced CS level in murine CS-producing tumors and increased infiltration of CD8+ T cells. When combined with immune checkpoint blockade or antigen-specific T cell transfer, 3\u03b2-OH-5-Chln suppressed the growth of CS-producing tumors. These results demonstrate that pharmacological inhibition of SULT2B1b can promote antitumor immunity through suppressing CS-mediated T cell exclusion.<\/p>\n\n\n\n<p><a href=\"https:\/\/researchmap.jp\/-kunimura-\/published_papers\/36729302\">https:\/\/researchmap.jp\/-kunimura-\/published_papers\/36729302<\/a><\/p>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"773\" height=\"449\" src=\"https:\/\/bioreg.kyushu-u.ac.jp\/labo\/iden\/wp\/wp-content\/uploads\/2025\/04\/\u30b9\u30af\u30ea\u30fc\u30f3\u30b7\u30e7\u30c3\u30c8-2025-07-03-13.08.30.jpg\" alt=\"\" class=\"wp-image-265\" style=\"width:670px;height:auto\" srcset=\"https:\/\/bioreg.kyushu-u.ac.jp\/labo\/iden\/wp\/wp-content\/uploads\/2025\/04\/\u30b9\u30af\u30ea\u30fc\u30f3\u30b7\u30e7\u30c3\u30c8-2025-07-03-13.08.30.jpg 773w, https:\/\/bioreg.kyushu-u.ac.jp\/labo\/iden\/wp\/wp-content\/uploads\/2025\/04\/\u30b9\u30af\u30ea\u30fc\u30f3\u30b7\u30e7\u30c3\u30c8-2025-07-03-13.08.30-300x174.jpg 300w, https:\/\/bioreg.kyushu-u.ac.jp\/labo\/iden\/wp\/wp-content\/uploads\/2025\/04\/\u30b9\u30af\u30ea\u30fc\u30f3\u30b7\u30e7\u30c3\u30c8-2025-07-03-13.08.30-768x446.jpg 768w\" sizes=\"auto, (max-width: 773px) 100vw, 773px\" \/><\/figure>\n","protected":false},"featured_media":253,"template":"","service_category":[8],"class_list":["post-252","service","type-service","status-publish","has-post-thumbnail","hentry","service_category-original-paper"],"_links":{"self":[{"href":"https:\/\/bioreg.kyushu-u.ac.jp\/labo\/iden\/wp-json\/wp\/v2\/service\/252","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/bioreg.kyushu-u.ac.jp\/labo\/iden\/wp-json\/wp\/v2\/service"}],"about":[{"href":"https:\/\/bioreg.kyushu-u.ac.jp\/labo\/iden\/wp-json\/wp\/v2\/types\/service"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bioreg.kyushu-u.ac.jp\/labo\/iden\/wp-json\/wp\/v2\/media\/253"}],"wp:attachment":[{"href":"https:\/\/bioreg.kyushu-u.ac.jp\/labo\/iden\/wp-json\/wp\/v2\/media?parent=252"}],"wp:term":[{"taxonomy":"service_category","embeddable":true,"href":"https:\/\/bioreg.kyushu-u.ac.jp\/labo\/iden\/wp-json\/wp\/v2\/service_category?post=252"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}