The Ohkawa Laboratory at the Medical Institute of Bioregulation, Kyushu University, has developed PECAbs (Precise Emission Canceling Antibodies) with cleavable fluorescent labels. PECAbs enable high-specificity sequential imaging using hundreds of antibodies, allowing for the reconstruction of spatiotemporal dynamics of signal transduction pathways. Furthermore, by combining this approach with continuous single-molecule RNA-FISH, it is possible to effectively classify cells within human tissues and identify their signal activation states. The PECAb system functions as a comprehensive platform for analyzing complex cellular processes.
This content has been published in the journal Nature Communications.
Title: Precise immunofluorescence canceling for highly multiplexed imaging to capture specific cell states
Authors: Kosuke Tomimatsu, Takeru Fujii, Ryoma Bise, Kazufumi Hosoda, Yosuke Taniguchi, Hiroshi Ochiai, Hiroaki Ohishi, Kanta Ando, Ryoma Minami, Kaori Tanaka, Taro Tachibana, Seiichi Mori, Akihito Harada, Kazumitsu Maehara, Masao Nagasaki, Seiichi Uchida, Hiroshi Kimura, Masashi Narita & Yasuyuki Ohkawa
Nature Communications, 15, 3657 (2024)