当分野の歴史
生医研生化学部門は昭和57年4月の研究所の改組前は九州大学医学部付属癌研究施設化学部門（昭和32年〜昭和57年）であり、改組に伴い生体防御医学研究所の生化学部門として発足した。その間生医研の初代所長である遠藤英也教授のもとで、発癌の機構を主として発癌物質の作用機構や癌化に伴う遺伝子発現の異常の問題を中心に研究が行われた。平成元年3月に遠藤教授が停年退官され、平成2年1月に関口睦夫教授が就任した。関口教授のもとでは、細胞癌化の問題を遺伝子の突然変異のレベルへ掘り下げて研究が行われた。そして平成9年8月より第3代教授として中別府雄作教授が着任した。
平成１３年４月１日付けの研究所改組にともない、生化学部門は廃止され、新たに個体機能制御学部門・脳機能制御学分野として再編された。現在、活性酸素によるゲノム傷害とその防御機構という観点から「がん」から「神経変性死」に着目して研究を進めている。
歴代教授および主要論文
遠藤英也教授 1982年4月-1989年3月
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M. (1988) Reactivation of rat insulin-like growth factor II gene during
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Goto, K., Endo, H. and Fujiyoshi, T. (1988) Cloning of the sequences expressed
abundantly in established cell lines: identification of a cDNA clone highly
homologous to S-100, a calcium binding protein J. Biochem. 103, 48-53
Ueno, T., Takahashi, K., Matsuguchi, T., Endo, H. and Yamamoto, M. (1987)
A new leader exon identified in the rat insulin-like growth factor II
gene Biochem. Biophys. Res. Commun. 148, 344-349
Ichiyoshi, Y., Endo, H. and Yamamoto, M. (1987) Length polymorphism in
the 3' noncoding region of rat hepatic alpha 2u-globulin mRNAs. Biochim.
Biophys. Acta 91043-51
Kohnoe, S., Maehara, Y. and Endo, H. (1987) A systematic survey of repetitive
sequences abundantly expressed in rat tumors. Biochim. Biophys. Acta 909,
107-114
Suzuki, N., Fujiyoshi, T., Maehara, Y., Takahashi, K., Yamamoto, M. and
Endo, H. (1986) A new family of LTR-like sequences abundantly expressed
in rat tumors. Nucleic Acids Res. 14,9271-9289
Miyahara, M., Sumiyoshi, H., Yamamoto, M. and Endo, H. (1985) Strand specific
transcription of satellite DNA I in rat ascites hepatoma cells. Biochem.
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Maehara, Y., Fujiyoshi, T., Takahashi, K., Yamamoto, M. and Endo, H. (1985)
1.5 kb mRNA abundantly expressed in rat tumors encodes a 37 kilodalton
protein in vitro Biochem. Biophys. Res. Commun., 131, 800-805
Yamamoto, M., Maehara, Y., Takahashi, K. and Endo, H. (1983) Cloning of
sequences expressed specifically in tumors of rat. Proc. Natl Acad. Sci.
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関口睦夫教授 1990年1月-1996年3月
Iwakuma, T., Sakumi, K., Nakatsuru, Y., Kawate, H., Igarashi, H., Shiraishi,
A., Tsuzuki, T., Ishikawa, T. and Sekiguchi, M. (1997) High incidence
of nitrosamine-induced tumorigenesis in mice lacking DNA repair methyltransferase.
Carcinogenesis 18, 1631-1635.
Sakumi, K., Shiraishi, A., Shimizu, S., Tsuzuki, T., Ishikawa, T. and
Sekiguchi, M. (1997) Methylnitrosourea-induced tumorigenesis in MGMT gene-knockout
mice. Cancer Res. 57, 2415-2418.
Oda, H., Nakabeppu, Y., Furuichi, M., and Sekiguchi, M. (1997). Regulation
of Expression of the Human MTH1 Gene Encoding 8-Oxo-dGTPase: Alternative
Splicing of Transcription Products. J. Biol. Chem. 272 (28), 17843-17850.
Cai, J-P., Kawate, H., Ihara, K., Yakushiji, H.,. Nakabeppu, Y., Tsuzuki,
T., Sekiguchi, M. (1997). Significance of the conserved amino acid sequence
for human MTH1 protien with antimutator activity. Nucleic Acids Res. 25
(6), 1170-1176.
Igarashi, H., Tsuzuki, T., Kakuma, T., Tominaga, Y. and Sekiguchi, M.
(1997) Organization and expression of the mouse MTH1 gene for preventing
transversion mutation. J. Biol. Chem. 272, 3766-3772.
Taketomi, A., Nakabeppu, Y., Ihara, K., Hart, D. J., Furuichi, M. and
Sekiguchi, M. (1996). Requirement of two conserved cysteine residues of
Ada protein of Escherichia coli for transactivation of ada promoter. Mol.
Gen. Genet. 250, 523-532.
Tsuzuki, T., Fujii, Y., Sakumi, K., Tominaga, Y., Nakao, K., Sekiguchi,
M., Matsushiro, A., Yoshimura, Y. and Morita, T. (1996) Targeted disruption
of the Rad51 gene leads to lethality in embryonic mice. Proc. Natl. Acad.
Sci. USA 93, 6236-6240.
Tsuzuki, T., Sakumi, K., Shiraishi, A., Kawate, H., Igarashi, H., Iwakuma,
T., Tominaga, Y., Zhang, S., Shimizu, S., Ishikawa, T., Nakamura, K.,
Nakao, K., Katsuki, M. and Sekiguchi, M. (1996) Targeted disruption of
the DNA repair methyltransferase gene renders mice hypersensitive to alkylating
agent. Carcinogenesis 17, 1215-1220.
Tajiri, T., Maki, H. and Sekiguchi, M. (1995) Functional cooperation of
MutT, MutM and MutY proteins in preventing mutations caused by spontaneous
oxidation of guanine nucleotide in Escherichia coli. Mutat. Res. 336,
257-267.
Furuichi, M., Yoshida, M. C.,oda, H., Tajiri, T., Nakabeppu, Y., Tsuzuki,
T. and Sekiguchi, M. (1994). Genomic structure and chromosome location
of the human mutT homologue gene hMTH1 encoding 8-oxo-dGTPase for prevention
of A:T to C:G transversion. Genomics 24, 485 -490.
Oda, S., Nishida, J., Nakabeppu, Y. and Sekiguchi,M. (1995). Stabilization
of cyclin E and cdk2 mRNAs at G1/S transition in Rat-1A cells emerging
from the G0 state. Oncogene 10, 1341-1351.
Ishibashi, T., Nakabeppu, Y., and Sekiguchi, M. (1994). Artificial control
of nuclear translocation of DNA repair methyltransferase. J. Biol. Chem.
269, 7645-7650.
Sakumi, K., Furuichi, M., Tsuzuki, T., Kakuma, T., Kawabata, S., Maki,
H., Sekiguchi, M. (1993) Cloning and expression of cDNA for human enzyme
that hydrolyzes 8-oxo-dGTP, a mutagenic substrate for DNA synthesis. J.
Biol. Chem. 268, 23524-23530.
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