第721回 生医研セミナー(多階層生体防御システム研究拠点)


下記の通り、杉井先生によるセミナーを開催致します。

杉井先生は、これまでコレステロールの生合成と輸送、脂肪細胞の分化などの領域を代表する独創的な研究を推進されてきています。特に脂肪組織由来幹細胞に関する研究では大きく貢献され、国際的にも非常に高く評価されておいでです。また、本領域研究の面白さに加えて自身の院生時代も含めた海外での研鑽など、意見交換等と共に語って頂きます。 多くの方々のご来聴をお待ちしております。

演題

Adipose-derived Stem Cells: Potential for Metabolic Reprogramming.
(Seminar in English)

演者

杉井 重紀 博士 (Shigeki Sugii, Ph.D.)
A*STARシンガポールバイオイメージング研究所 グループリーダー、
Duke-NUS医学系大学院 助教授 

日時

2016年 9月12日(月) Sep. 12 (Mon), 2016
10:00~12:00

場所

病院キャンパス内 総合研究棟1階 105
以下の地図の1番です。
(https://www.kyushu-u.ac.jp/f/28545/hospital_jp.pdf)

Seminar Room 105, 1F, Biomedical Research Station
No.1 on the following linked map.
(https://www.kyushu-u.ac.jp/f/28551/hospital_en.pdf)

要旨

Adipose tissue is an expandable and readily attainable source of proliferating, multipotent adipose-derived stem cells (ASCs), holding great therapeutic potentials. However, more work is necessary to fully understand biological properties of ASCs and explore translational approaches by using or targeting ASCs. By comprehensive image-based high content screening, we identified novel fat-depot specific cell surface markers, CD10 and CD200, which can predict how well ASCs can differentiate into mature functional adipocytes. Through another study on whole genome-wide gene expression analyses, we identified novel pathways of retinoid metabolism and oxidative stress. High level of retinoic acid negatively affects early stage of adipogenic differentiation of ASCs, which can be reversed by antagonism of retinoic acid receptors. Similarly it was found that high oxidative stress associated with ageing or visceral obesity affects ASC’s ability for differentiation, proliferation, migration and/or senescence. Treatment with anti-oxidants was found to be effective in reducing reactive oxygen species and improving these ASC properties. Collectively, these results suggest that stem cells can be the cellular target for improving quality of fat tissue and adipocytes through use of specific cell surface markers, modulating RA pathway, or reversal of oxidative stress. These molecular markers and factors may be useful for future metabolic reprogramming studies in different approaches: bioimaging, screening for improved adipocyte development, or reprogramming into induced pluripotent stem (iPS) cells.

参考文献

  • Takeda, K., Sriram, S., Chan, X.H.D., Ong, W.K., Yeo, C.R., Tan, B., Lee, S.A., Kong, K.V., Hoon, S., Jiang, H., Yuen, J.J., Perumal, J., Agrawal, M., Vaz, C., So, J., Shabbir, A., Blaner, W.S., Olivo, M., Han, W., Tanavde, V., Toh, S.A., and Sugii, S. (2016) Retinoic Acid Modulates Visceral-Specific Adipogenic Defects of Human Adipose-derived Stem Cells. Diabetes 65, 1164-1178.
  • Ong, W.K., Tan, C.S., Chan, K.L., Goesantoso, G.G., Chan, X.H.D., Chan, E., Yin, J., Yeo, C.R., Khoo, C.M., So, J., Shabbir, A., Toh, S.A., Han, W., and Sugii, S. (2014) Identification of Specific Cell Surface Markers of Adipose-derived Stem Cells from Subcutaneous and Visceral Fat Depots. Stem Cell Reports 2, 171-179.
  • Lim, M.H., Ong, W.K., and Sugii, S. (2014) Current Landscape of Adipose-derived Stem Cells in Clinical Applications. Expert Rev. Mol. Med. 16, e8.
  • Sugii, S., Kida, Y., Berggren, W.T., and Evans, R.M. (2011) Feeder-Dependent and Feeder-Independent iPS Cell Derivation from Human and Mouse Adipose Stem Cells. Nature Protoc. 6, 346-358.

連絡先

生体防御医学研究所 オルガネラホメオスタシス研究室
藤木 幸夫 092-642-4232 (秘書 6341)