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第713回　生医研セミナー(多階層生体防御システム研究拠点)

下記の通り、Michael Bassik, Ph.D.( Assistant Professor, Stanford University )のセミナーを二部構成で行なうことになりました。

Bassik先生は、初めて”systematic genetic interaction maps in human cells”の確立に成功するなど世界的な研究成果をこれまで数多く発信されてきています。最近では、”a double sgRNA platform”の開発に成功していて国際的にも高く評価されています。

第２部のみの参加も歓迎します。多くの方々のご来聴をお待ちしております。

日時

2016年 3月 18日（金）14：30～17：20

場所

馬出キャンパス 総合研究棟１階 102講義室
以下の地図の1番です。
（http://www.kyushu-u.ac.jp/access/map/hospital/hospital.html）

演者

Michael Bassik, Ph.D.
Assistant Professor, Department of Genetics, Stanford University, USA

【第１部】14:30～16:00

演　　題Parallel shRNA and CRISPR/Cas9 Screens Reveal Biology of Stress Pathways and Identify Novel Drug Targets.
要　　旨We have developed high-complexity shRNA libraries (25 shRNAs/gene) that greatly reduce false negatives/false positives, and have adapted these libraries to knock down gene pairs to perform systematic genetic interaction maps in mammalian cells. We have used these maps to study ER-trafficking toxins, and identified novel protein complexes as well as insights into retrograde trafficking. We are now using this strategy together with the CRISPR/Cas9 system to study stress signaling more globally, as well as to identify novel drug targets. In recent work, we have used parallel shRNA and CRISPR/Cas9 screening to explore the cellular response to oxidative stress, and have identified the target and mechanism of action of a novel host-targeting antiviral drug.
参考文献
  1. Bassik MC, Lebbink RJ, Churchman LS, Ingolia NT, Patena W, Leproust EM, Schuldiner M, Weissman JS, McManus MT. Rapid creation and quantitative monitoring of high coverage shRNA libraries. Nat Methods. 2009 May 17; (6) 443-5.
  2. He C*, Bassik MC*, Moresi V, Sun K, Wei Y, Zou Z, An Z, Loh J, Fisher J, Sun Q, Korsmeyer S, Packer M, May HI, Hill JA, Virgin HW, Gilpin C, Xiao G, Bassel-Duby R, Scherer PE, Levine B. Exercise-induced BCL2-regulated autophagy is required for muscle glucose homeostasis. Nature. 2012 Jan 18;481(7382):511-5.
  3. Bassik MC, Kampmann M, Lebbink RJ, Wang S, Hein MY, Poser I, Weibezahn J, Horlbeck MA, Mann M, Hyman AA, Leproust EM, McManus MT, and Weissman JS. A Systematic Mammalian Genetic Interaction Map Reveals Pathways Underlying Ricin Susceptibility. Cell. 2013 Feb 14; 152: 909–922.
  4. Kampmann M*, Bassik MC*, Weissman JS. Functional genomics platform for pooled screening and generation of mammalian genetic interaction maps. Nat Protoc. 2014 Aug;9(8):1825-47. doi: 10.1038/nprot.2014.103.
  5. Deans RM, Morgens DW, Ökesli A, Pillay S, Horlbeck MA, Kampmann M, Gilbert LA, Li A, Mateo R, Smith M, Glenn JS, Carette JE, Khosla C*, and Bassik MC*. Parallel shRNA and CRISPR-Cas9 screens enable antiviral drug target identification. Nat Chem. Biol. 2016, in press.

【第２部】16:20～17:20

演　　題「Progress100:若手研究者育成フォーラム」
Collaboration and lots of help: my career path in academia and starting a new lab
概　　要I will describe what for me were important lessons I learned during my scientific training, as well as some thoughts about starting a new lab and science in the US. Free and open discussion with the audience.

連絡先

生体防御医学研究所　
オルガネラホメオスタシス研究室
藤木　幸夫　092-642-4232 (秘書　6341)

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